SPAD Elevates Spatiotemporal Resolution in Conventional Microscopes
GENOA, Italy, Feb. 23, 2024 — Image scanning microscopy (ISM), a superresolution technique enabled by the advent of fast and compact detector arrays, provides better signal-to-noise ratio (SNR), optical sectioning, and spatial resolution better than that of a traditional confocal microscope. The lateral resolution of the ISM image can surpass Abbe’s limit by up to a factor of two. However, these advantages are achieved by exploiting only spatial information; modern fluorescence bioimaging can be further enriched