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Hot Flies Produce Cool Results

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ITHACA, N.Y., Sept. 25, 2006 -- Using multiphoton fluorescence microscopy, researchers have for the first time watched chromosomes change form to activate their genes to synthesize key proteins in fruit fly cells. The advance could be a significant step toward understanding the basic processes that underlie gene expression in living tissue. The discovery was the result of cross-disciplinary collaboration between Cornell University applied physics professor Watt W. Webb, who pioneered the microscopy technique, and John Lis, the university's Barbara McClintock Professor of Molecular Biology and Genetics. Jie Yao, who...Read full article

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    Published: September 2006
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    photobleaching
    Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
    Cornelldrosophilafluorescencefruit fliesgene expressionHSFhsp70MicroscopyMPMmultiphoton microscopyNews & FeaturesphotobleachingWebbYao

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