Gold nanoparticles have been strategically attached to proteins to form sheets of protein-gold arrays. The nanoparticles and methods to create nanoparticle-protein complexes can be used to help decipher protein structures, to identify functional parts of proteins and to "glue" together new protein complexes. Catalysts for converting biomass to energy and precision "vehicles" for targeted drug delivery are among applications envisioned by researchers at the US Department of Energy's Brookhaven National Laboratory who demonstrated the results (to be published this month in the journal Angewandte Chemieinclude).A cryo-electron micrograph showing a single layer of evenly spaced enzyme structures (colorful "wheels") interspersed with gold nanoparticles (magenta). Constructing such regular arrays of biomolecules might help develop high efficiency electrodes for biofuels or improve the resolution of cryo-electron microscopy in structural biology. (Photo courtesy BNL Media & Communications Office) "Our study demonstrates that nanoparticles are appealing templates for assembling functional biomolecules with extensive potential impact across the fields of energy conversion, structural biology, drug delivery and medical imaging," said lead author Minghui Hu, a postdoctoral student working with James Hainfeld, Raymond Brinas, Luping Qian and Elena Lymar in the biology department at Brookhaven Lab. In one application, the new research showed that gold nanoparticles -- on the order of billionths of a meter -- can enhance scientists' ability to decipher the structures and functionally important regions of protein molecules, the workhorses that carry out every function of living cells and which can often lead to disease if they dysfunction. With added nanoparticles, the signal-to-noise ratio and resolution of an imaging technique known as cryo-electron microscopy were significantly increased. This method might enable analysis of small biological macromolecules and complexes that are currently intractable to analyze by cryo-electron microscopy or x-ray crystallography. Left to right: James Hainfeld, Luping Qian and Minghui Hu (Photo courtesy BNL Media & Communications Office) In the field of energy conversion, scientists have been searching for efficient ways to convert organic fuels such as ethanol into electricity using catalytic electrodes. But making single layers of densely packed enzymes, the functional part of such catalytic electrodes, has been a challenge, the lab said in a statement. The new research shows that precisely engineered gold nanoparticles can be used to "glue" enzymes together to form oriented and ordered single layers, and that these monolayers are mechanically stable enough to be transferred onto a solid surface such as an electrode. The scientists attached gold nanoparticles to an enzyme complex that helps drug-resistant tuberculosis bacteria survive, which has been studied by Brookhaven Lab biologist Huilin Li. They suggest that gold nanoparticles might also be tailored to inactivate this enzyme complex, thereby thwarting drug-resistant tuberculosis (TB) -- a research avenue they may explore in future studies. In another part of the study, they used proteins found on the surface of adenovirus, a virus that causes the common cold. Previous studies by Broookhaven's Paul Freimuth have characterized how this virus binds to the human cells it infects and have suggested that modified forms of adenovirus could be used as vehicles to deliver drugs to specific target cells, such as those which make up tumors. One key to this approach would be to enhance strong binding to the target cells. Toward that end, Hu and Hainfeld's group attached multiple viral proteins to the gold nanoparticles. Such constructs should have increased binding affinity for target cells, and their larger size should extend blood residence time for improved drug delivery. The scientists said the biggest challenge in their research was to synthesize size-controllable nanoparticles coated with organic molecules designed to react with specific protein sites. Hu explained the steps: "First, we design the specific interactions between gold nanoparticles and the proteins by coating the gold nanoparticles with functional organic molecules using a biocompatible linker. Then we add a genetically engineered sequence of peptides, called a 'tag,' to the protein molecule, which acts as the binding site for the gold nanoparticles. Finally, we incubate the nanoparticles with the protein solution to allow the nanoparticles and proteins to bind, transfer the solution onto a transmission electron microscopy grid, and analyze the complexes using state-of-the-art electron microscopes." For more information, visit: www.bnl.gov