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Detecting viral DNA

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Surface hybridization advances possible clinical screening technique

Gary Boas

The DNA-based human papilloma virus attacks skin and mucous membranes, causing warts or infections that can lead to precancerous lesions. Clinicians often use methods such as target amplification -- in particular, polymerase chain reaction (PCR) -- to detect such viral DNA. These well-developed techniques offer high efficiency, but they also can yield high numbers of false-positive and false-negative results. In PCR, for example, the presence of small contaminants can lead to false-positive results. Single-molecule spectroscopy does not require amplification, and thus is an attractive...Read full article

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    Published: January 2008
    Basic ScienceBiophotonicsDNApolymerase chain reactionResearch & Technology

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