Part of the “Methods in Molecular Biology” series, the second edition of Protocols for Nucleic Acid Analysisby Nonradioactive Probes presents the methodologies using new applications. The 336-page volume offers chapters on nucleic acid extractions, preparing nucleic acid blots and labeling nucleic acids with nonradioactive haptens. Fluorescence techniques such as real-time polymerase chain reaction and microarrays are included. Two ways of detecting nonradioactive probes are discussed: indirect methods using a labeled probe or direct methods detecting the labeled nucleic acid. The protocols follow the series format, offering step-by-step laboratory instructions, an introduction to the principles of the techniques, lists of required equipment and reagents, and suggestions on troubleshooting and avoiding known problems. Elena Hilario and John F. MacKay, eds.; Humana Press, a part of Springer Science+Business Media, Totowa, N.J., 2007; $103.60; €75.
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