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BioPhotonics
May 2007
CoolLed has launched a revised Web site to focus on the application of its precisExcite LED light source for fluorescence microscopy. The resource explains the benefits of LED excitation of fluorophores, which include extended lifetime, ease of use and better control. It notes that, with the source, there is no need for neutral density filters, no need to align the lamp, no need to record lamp usage and no degradation of performance over time. It includes downloadable application notes on such topics as users’ experiences with the light source for live-cell imaging. Related time-lapse videos show epithelial cells derived from the lining of the human airway. The portal also includes sections on news, filters, endorsements and distributors, along with a photo gallery and an explanation of fluorescence microscopy.



GLOSSARY
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
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