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  • Labeling, detection

Apr 2007
Invitrogen.jpg“BioProbes 52,” the March issue of a newsletter published by Molecular Probes Inc., includes articles on emerging technologies, such as Click-iT glycoprotein profiling reagents, a Premo cameleon calcium sensor for listening in on cellular communications, and Saivi Alexa Fluor antibody/protein labeling kits for in vivo animal imaging. A section on imaging analysis includes a discussion on the use of fluorescence and chromogenic in situ hybridization to visualize RNA and DNA targets, as well as coverage of fluorescent probes for two-photon microscopy, with applications in neuroscience and other areas. Bioarsenical reagents for live-cell imaging and protein labeling also are covered in the 40-page document, which includes numerous charts, images and references.

The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
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