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Confocal microscopy

BioPhotonics
Feb 2007
Springer2.jpgThe Atlas of Confocal Laser Scanning In-Vivo Microscopy in Ophthalmology discusses the principles of confocal in vivo microscopy, including slit-scanning techniques, the basics of image formation and confocal fluorescence microscopy. Written for ophthalmologists and other physicians interested in the techniques, the resource demonstrates normal in vivo anatomy of the cornea, limbus and conjunctiva and quantifies cellular structures using cell-density calculations. It establishes correlations between novel optical sections of various diseases of the ocular surface and clinical findings. With 692 illustrations, many of which are in color, the 212-page resource covers nonophthalmological applications as well as promising ones such as glaucoma surgery and alterations of the cornea in contact lens wearers. References and an index are provided. R.F. Guthoff, C. Baudouin and J. Stave; Springer, New York, 2006; $89.95.


GLOSSARY
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
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