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Fluorescence lifetime shows temperatures of flowing liquids

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Understanding temperature distribution can help optimize chemical processes on microchips

Lynn M. Savage

Many biological analytical processes would benefit from miniaturization, but some —such as DNA amplification through polymerase chain reaction (PCR) — depend on homogeneous temperature gradients to optimize the chemical or molecular reactions. Putting PCR on a microfluidic chip, for example, should improve its efficiency, response and cost; however, achieving consistent temperatures through a liquid sample in a chip requires a finely tuned system for determining what the temperatures are at any given point in space and time. Existing temperature-monitoring technology —...Read full article

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    Published: May 2006
    Basic ScienceBiophotonicsMicroscopyResearch & Technology

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