BELFAST, Northern Ireland, March 4, 2011 — Andor Technology plc has launched the TuCam, a state-of-the-art adapter for simultaneous two-camera macro- and microscopic imaging.
Suited to fast, concurrent detection of two different fluorophores in experiments such as colocalization Förster resonance energy transfer and ratiometric ion signaling, it is compatible with the company’s low-light imaging cameras, including iXon3 and Luca electron-multiplying CCDs, the Clara interline CCD and the Neo sCMOS model. Bypass mode and first surface mirror enable switching between cameras.
It can be configured for simultaneous imaging from two similar cameras, or as a switch between camera models with different capabilities.
The robust design ensures good optical and mechanical stability, and ease of alignment via kinematic cassettes. Available in C- and CSUX-mount versions and offering a variety of beamsplitting optics, including wavelength and polarization filter sets, the adapter also features achromatic lenses that enable imaging from 425 to 700 nm with minimal adjustment, high transmission, low distortion and good registration between camera channels. A 22-mm aperture certifies compatibility with large-format sensors for imaging wide fields of view.
When integrated into the proprietary Revolution microscopy system, it can be used for real-time confocal and total internal reflection fluorescence where multichannel or polarization imaging is required. When coupled with the iXon3, it can perform dual-color single-molecule and biplane 3-D superresolution imaging.
Transmission is >96% from 425 to 700 nm.
A variety of camera tubes and lenses provide magnifications of 1×, 2×, 1.5× and 2× in each arm of the adapter. A filter wheel can be integrated at the input to enable prefiltering of the desired emission band.
For laboratory use, the selected cameras can be detached and used individually on different optical setups as required, then realigned onto the TuCam for dual-wavelength imaging.
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