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Hamamatsu Corp. - Earth Innovations LB 2/24

New sCMOS vs. Current Microscopy Cameras

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Dr. Colin Coates, Andor Technology PLC

Since the launch in late 2010 of imaging cameras based on a new 5.5-megapixel scientific CMOS (sCMOS) sensor, there has been much speculation about whether sCMOS will become a technology replacement for interline CCD and electron-multiplying CCD (EMCCD) cameras, in many ways considered the current gold standard for low-light fluorescence microscopy and other bioimaging techniques. Coming from the unique market position of manufacturing all of the aforementioned camera types, we provide here a comparison of these sensitive imaging technologies. Technology overview The small-pixel...Read full article

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    Published: May 2011
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    electron multiplying ccd
    A CCD device in which a solid-state electron multiplying register has been added to the end of the normal serial register. The electron multiplying register allows weak signals to be multiplied before readout noise is added by the output amplifier.
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    CCDCMOSAndor Technologysuperresolutionbio-imagingBiophotonicscamerasColin Coateselectron multiplying CCDEMCCDEuropeFeaturesfluorescence microscopyImagingindustrialinterline CCDMicroscopypixel sizeScientific CMOSsCMOSSensors & Detectorssignal to noiseSNRsuperresolution microscopy

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