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The (Never Ending?) Search for Higher-Resolution Microscopy

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Lynn Savage, Features Editor, [email protected]

Can optical microscopy further shred the Abbe diffraction limit? Is it important to even try? Until about two decades ago, optical microscopists were stuck viewing their samples with a resolution no better than 200 nm. Now, well-funded labs have access to imaging down to 20 nm – and sometimes better still. Super-resolution optical techniques, in which fluorescent particles are manipulated into providing imaging resolutions far less than 200 nm, are primarily used in imaging and photolithography. Any technology that seeks to further improve upon the long-defeated diffraction limit...Read full article

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    Published: July 2011
    Glossary
    photolithography
    Photolithography is a key process in the manufacturing of semiconductor devices, integrated circuits, and microelectromechanical systems (MEMS). It is a photomechanical process used to transfer geometric patterns from a photomask or reticle to a photosensitive chemical photoresist on a substrate, typically a silicon wafer. The basic steps of photolithography include: Cleaning the substrate: The substrate, often a silicon wafer, is cleaned to remove any contaminants from its surface. ...
    stochastic optical reconstruction microscopy
    Stochastic optical reconstruction microscopy (STORM) is a super-resolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional optical microscopy. It falls under the category of single-molecule localization microscopy (SMLM) methods. STORM was first introduced in 2006 and has since become a powerful tool in biological research for visualizing fine details of cellular structures. The key principle behind STORM involves...
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    Basic ScienceBiophotonicsCaliforniaCarl Zeiss MicroImaging LLCcell dynamicscell imagingConsumerdiffraction limitDuncan McMillanenergyFeaturesFiltersfluorescent particlesfluorophoresground state depletion microscopy–individual molecule returnGSDIMindustriallensesMicroscopymolecular imagingNew YorkNikon Instruments Inc.optical microscopyOpticsPALMphotoactivated localization microscopyphotolithographyPrashant PrabhatproteinsSemrock Inc.STEDStephen T. Rossstimulated emission depletionstochastic optical reconstruction microscopySTORMsuper-resolution microscopy techniquessuperresolutionUniversity of California BerkeleyYuan Wang

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