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  • Glowing Molecules Turn On, Off to Visualize Cell Activity

Photonics.com
Aug 2012
CORAL GABLES, Fla., Aug. 17, 2012 — Using UV light to switch fluorescent molecules on and off in aqueous environments could help scientists develop better fluorescent probes for biomedical research.

The molecules first must be strategically trapped inside water-soluble particles, which isn't new; water-soluble particles have been used in previous studies to bring organic molecules into water. What is new in this system, developed by University of Miami scientists, is the addition of the photoswitching mechanism.

"Finding a way to switch fluorescence inside cells is one of the main challenges in the development of fluorescent probes for bioimaging applications," said chemistry professor Francisco Raymo. "Our fluorescent switches can be operated in water efficiently, offering the opportunity to image biological samples with resolution at the nanometer level."


UV light now can switch fluorescent molecules on and off in aqueous environments. Here, live cells have been incubated with the polymer nanoparticles. The green color is the fluorescence coming from the molecules trapped within the nanoparticles. (Images: Francisco Raymo, University of Miami)

Because fluorescent molecules are not water soluble, Raymo and his team created their system by embedding fluorescent molecules in synthetic water-soluble polymer nanoparticles, which serve as transport vehicles into living cells. Once inside cells, the fluorescence of the molecules trapped inside the nanoparticles can be turned on and off under optical control.

"The polymers can preserve the properties of the fluorescent molecules and at the same time assist the transfer of the molecules into water," Raymo said. "It's a bit like having a fish in a bowl, so the fish can carry on with its activities in the bowl, and the whole bowl can be transferred into a different environment."

The new system is faster and more stable than current methods. The fluorescent molecules glow when exposed simultaneously to UV and visible light and revert back to their original nonluminous state in less than 10 µs after UV light is removed. Using synthetic molecules enables the system to overcome the natural wear that organic molecules endure from overexposure to UV light.


Francisco Raymo, a professor of chemistry at the University of Miami, developed a way to switch fluorescent molecules on and off within aqueous environments. The new technique could help scientists develop better fluorescent probes for biomedical research.

"The system can be switched back and forth between the fluorescent and nonfluorescent states for hundreds of cycles, without sign of degradation," Raymo said.

The system’s surface can be customized to help it attach to specific molecules of interest, enabling researchers to visualize structures and activity within cells, in real time, with unprecedented resolution.

The findings were reported online by Chemistry-A European Journal.

For more information, visit: www.umiami.edu


GLOSSARY
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
photonics
The technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. The science includes light emission, transmission, deflection, amplification and detection by optical components and instruments, lasers and other light sources, fiber optics, electro-optical instrumentation, related hardware and electronics, and sophisticated systems. The range of applications of photonics extends from energy generation to detection to communications and...
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