CENTER VALLEY, Pa., Oct. 12, 2012 — A multiphoton microscope system introduced by Olympus America Inc. allows researchers to achieve more sensitive, faster live-cell imaging deeper within tissue. The FluoView FV1200MPE laser scanning imaging system offers increased laser selection, cooled high-sensitivity detector technology and new software features for high-speed functional measurements.
The system offers new approaches for measuring rapidly changing fluorescent output, while reducing laser power, phototoxicity and photobleaching. Its scan head has anti-corrosive silver-coated galvo mirrors that increase light throughput.
It supports Newport Corp.’s Insight laser system for infrared imaging continuously from 690 to 1300 nm, enabling optimized excitation of longer-wavelength dyes as well as second- and third-harmonic-generation imaging. Dual simultaneous excitation wavelength configurations are available.
An optional GaAsP detector enhances sensitivity and minimizes electrical noise. The FV1200MPE is compatible with proprietary specialty objectives, including ScaleView.
Its multipoint mapping advanced software can measure fluctuations in groups of cells at speeds up to 101 Hz in a full field, with sequential position data output up to 50,000 Hz. Multichannel fluorescence measurements can be made for calcium ion fluctuation, optogenetic experiments with channelrhodopsin and halorhodopsin, stem cell research, patch clamping/electrophysiology, and studies of functional output over cells and cell networks.
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