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Laser device images, tracks cells, dye-free

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Ashley N. Rice, [email protected]

Without contrast dyes or fluorophores, a new device can image and track living cells’ reactions to various stimuli and create 3-D images of biological tissue at the nanoscale in just minutes. The system, developed by Yann Cotte and Fatih Toy of Ecole Polytechnique Fédérale de Lausanne (EPFL), combines holographic microscopy and computational image processing to obtain 3-D images of living cells from every angle at a resolution of less than 100 nm. Because the tissue can be imaged without using contrast dyes or fluorescents, foreign substances will not distort the...Read full article

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    Published: April 2013
    Glossary
    digital camera
    A camera that converts a collected image into pixels that are black or white digital or shades of gray. The digital data may then be manipulated to enhance or otherwise modify the resulting viewed image.
    holography
    Holography is a technique used to capture and reconstruct three-dimensional images using the principles of interference and diffraction of light. Unlike conventional photography, which records only the intensity of light, holography records both the intensity and phase information of light waves scattered from an object. This allows the faithful reproduction of the object's three-dimensional structure, including its depth, shape, and texture. The process of holography typically involves the...
    3-D live cell imagingBiophotonicsBioScancamerasChristian Depeursingecomputational image processingcontrast-free imagingdigital cameradye-free imagingEcole Polytechnique Fédérale de LausanneEPFLEuropeFatih Toyfluorescent-free imaginggrowing neuronholographyImagingliving tissue imagingMicroscopyNewsSwitzerlandYann CotteLasers

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