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Raman Scattering Sped Up for Microscopy

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Improvements to Raman spectroscopy using laser frequency combs allow multiple signals from different parts of a molecule — or even different molecules — to be monitored simultaneously using a single detector. The advance is seen as a major step toward the holy grail of real-time, label-free biomolecular imaging. For decades, biologists have attached fluorescent dye labels to certain proteins to distinguish them under a microscope. But such labels can alter the cell's function. Many molecules of interest have characteristic absorption spectra at mid-infrared wavelengths, but...Read full article

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    Published: October 2013
    Glossary
    metrology
    Metrology is the science and practice of measurement. It encompasses the theoretical and practical aspects of measurement, including the development of measurement standards, techniques, and instruments, as well as the application of measurement principles in various fields. The primary objectives of metrology are to ensure accuracy, reliability, and consistency in measurements and to establish traceability to recognized standards. Metrology plays a crucial role in science, industry,...
    laser spectroscopy
    That part of the science involved in the study of the theory and interpretation of spectra that uses the unique characteristics of the laser as an integral part in the development of information for analysis. Raman spectroscopy and emission spectroscopy are two areas where lasers are used.
    photodetector
    A photodetector, also known as a photosensor or photodiode, is a device that detects and converts light into an electrical signal. Photodetectors are widely used in various applications, ranging from simple light sensing to more complex tasks such as imaging and communication. Key features and principles of photodetectors include: Light sensing: The primary function of a photodetector is to sense or detect light. When photons (particles of light) strike the active area of the photodetector,...
    raman spectroscopy
    Raman spectroscopy is a technique used in analytical chemistry and physics to study vibrational, rotational, and other low-frequency modes in a system. Named after the Indian physicist Sir C.V. Raman who discovered the phenomenon in 1928, Raman spectroscopy provides information about molecular vibrations by measuring the inelastic scattering of monochromatic light. Here is a breakdown of the process: Incident light: A monochromatic (single wavelength) light, usually from a laser, is...
    spatial resolution
    Spatial resolution refers to the level of detail or granularity in an image or a spatial dataset. It is a measure of the smallest discernible or resolvable features in the spatial domain, typically expressed as the distance between two adjacent pixels or data points. In various contexts, spatial resolution can have slightly different meanings: Imaging and remote sensing: In the context of satellite imagery, aerial photography, or other imaging technologies, spatial resolution refers to the...
    BiophotonicsmetrologyBioScanEuropelabel-free imaginglaser frequency comblaser spectroscopyLasersMaterials & ChemicalsMax Planck Institute for Quantum OpticsMicroscopymid-infraredmoleculeMPQphotodetectorRaman scatteringRaman spectroscopyResearch & Technologyspatial resolutionspectroscopyTakuro IdeguchiTech PulseTest & MeasurementTheodor Hänsch

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