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Can Confocal Microscopes Compete with the Emergence of Superresolution?

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Super-corrected optics, increased sensitivity and faster throughput mean that confocal systems are revealing more biology in its natural context than ever before.

MARIE FREEBODY, CONTRIBUTING EDITOR, [email protected]

Like most things, confocal microscopes have improved with age. The technique dates back to 1955, when American cognitive scientist Professor Marvin Minsky proposed that the ideal microscope would eliminate all light rays not initially aimed at the focal point. Gathering the optical tools that were available to him at the time, Minsky fashioned the first confocal microscope using an arc lamp to illuminate the specimen and a low noise photomultiplier to measure the output. Instead of scanning mirrors, the specimen was sequentially moved to image point after point. Finally, the image was...Read full article

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    Published: January 2016
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    FeaturesBiophotonicsMarie Freebodyconfocal microscopearc lampscanning mirrorAmericas Europe/UKsuperresolution applicationssuperresolutionZeissEvotec AGOlympus Scientific Solutions Americasmultiphoton microscopyPerkin ElmerUC San DiegoMicroscopy

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