Ratiometry is a technique used in various scientific fields, particularly in analytical chemistry and biochemistry, to measure concentrations or quantities by comparing the intensity of two different signals or responses.
In ratiometry, instead of relying on absolute values of signals, researchers utilize the ratio of two signals or responses obtained from a sample. This ratio is often less affected by factors such as variations in sample volume, instrument settings, or environmental conditions, making it a robust method for quantitative analysis.
In fluorescence ratiometry, for example, scientists may measure the ratio of fluorescence intensities at two different wavelengths to determine the concentration of a fluorescent analyte. Similarly, in imaging techniques, ratiometric imaging involves capturing images at two different wavelengths and calculating the ratio of intensities to obtain quantitative information about the sample.
Overall, ratiometry provides a means to minimize errors and enhance the accuracy and reliability of measurements in various scientific applications.