ROS-Glo H2O2 Assay
MADISON, Wis., Oct. 8, 2013 — Promega Corp. has launched the ROS-Glo H2
, a nonhorseradish peroxidase (HRP)-dependent, plate-based bioluminescent assay designed to detect reactive oxygen species (ROS).
It measures the activity of enzymes that generate or eliminate H2
and enables small-molecule screening, including low false hit rate, signal stability and compatibility with liquid handling.
The homogeneous assay does not rely on a reaction catalyzed by HRP, which causes a high number of false hits. It also can be used to measure changes in the level of ROS by directly detecting H2
in cultured mammalian cells after two reagent additions, with no cell sample preparation, reducing variability and the number of cells required. Large compound libraries can be screened for their capacity to alter H2
levels in cultured cells or in enzymatic reactions.