WETZLAR, Germany, Sept. 12, 2013 — Leica Microsystems GmbH has released version 3.2 of LAS AF (Leica Application Suite Advanced Fluorescence), its proprietary software platform for wide-field and confocal microscopy systems for life sciences applications. Version 3.2 focuses on 3-D analysis, 3-D measurement and 3-D visualization. These functionalities render ground-state depletion data visible as volume-rendered images, adding a third dimension to superresolution images. Additional features include an image gallery for fast review of experiments, image selection and automatic detection of best-focused images. The 3-D analysis function allows users to measure various aspects of intracellular structures, such as the volumes and surfaces of nuclei, and the distances and angles between them. Individual 3-D objects can be measured with the interactive measurement tool. For automatic measurements, a wizard guides users through the 3-D analysis step by step, from image preprocessing, threshold adjustments, generation of a 3-D binary mask and definition of measurement parameters up to the generation of reports. Users can apply 3-D processing tools in any order.