Fluorescence filter sets for fluorophore analysis in fluorescence microscopy applications are available from Medway Optics Ltd. The sets comprise high-precision exciter and emitter filters that have a five-cavity design, plus dichroic longpass filters that reflect the excitation wavelengths and transmit the emission ones at a 45° angle of incidence. The emission filters are polished to high flatness and parallelism for blocking the excitation wavelengths, and the excitation filters are deeply blocked on both sides. Optical density of ≥5 is achieved at the exciter/emitter crossover, resulting in a good signal-to-noise ratio. The filters are available as sets or individually, in many sizes and with or without filter cubes, for Nikon, Olympus, Leica, Zeiss and other analytical microscopes.