Semrock Inc. has expanded its BrightLine family with single-band fluorescence filters for enhanced green fluorescent protein (GFP) and a variety of alternative fluorophores, including Alexa Fluor 488, calcein, fluorescein, Oregon green and rhodamine 110. The filters are made from proprietary hard-optical-coating technology and ion-beam sputtering, without any adhesives in the optical path. The company says that they offer high brightness, durability and a good signal-to-noise ratio. The filters are available on a volume OEM basis for most fluorescence microscopes and, optionally, with certified zero pixel shift, which is suitable for multiexposure applications. Applications include microarray and biochip readers, high-throughput screening, flow cytometry and fluorescence scanners.