Version 5.1 of Advanced Fluorescence Acquisition, an Image-Pro Plus plug-in module for acquiring and managing images in multiple dimensions, has been released by Media Cybernetics Inc. It includes additional functions for microscopists to automate and manage complex acquisition setup parameters, user feedback display and subsequent sorting into sets for analysis. Features include customizable macros, enhanced time-lapse tools, and a tool that records the position, zoom and scroll of various channels in their acquisition and saves the layout. Color composite images of multichannel experiments can be captured. The module is available as part of the Image-Pro multidimensional acquisition and Image-Pro advanced microscopy suites.