Carl Zeiss MicroImaging GmbH offers version 4.6 of its AxioVision software for digital microscopy in biomedicine and materials applications. Supporting the Axio Observer microscopes with Zeiss incubation modules, it features new and improved functions for recording, archiving, processing, analyzing and reconstructing images. It offers modules for topography, physiology, online measurement and digital high-speed recording. It includes a wavelet algorithm to calculate extended depth-of-focus images at high quality, and it provides perspective equalization on Z-stack images taken with stereomicroscopes. The Inside 4D mode allows the simultaneous display of transparency and surface render methods. The section plane mode enables “looking into” 3-D structures.