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TCSPC microscopy

BioPhotonics
Jun 2008
HORIBA ScientificRequest Info
 
Horiba-Yvon.jpgHoriba Jobin Yvon Inc. has introduced the DynaMic system designed to apply time-resolved fluorescence spectroscopy on the micron scale. The filter-based confocal instrument adapts time-correlated single-photon-counting (TCSPC) lifetime components directly to a microscope to achieve high sensitivity for fluorescence lifetime measurement and intensity mapping. Its wavelength range is from the deep UV to the VIS (240 to 850 nm). Suitable for applications such as intrinsic protein fluorescence measurement, it incorporates deep-UV pulsed LEDs (280, 295 and 340 nm) and offers a dynamic range covering lifetimes from <100 ps to 100 μs. Among other features, the device offers rapid data acquisition and plug-and-play components.


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GLOSSARY
fluorescence spectroscopy
The spectroscopic study of radiation emitted by the process of fluorescence.
microscope
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
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