Version 4.6 of the AxioVision software for digital microscopy has been released by Carl Zeiss MicroImaging GmbH. Suitable for biomedicine and materials applications, the software features new functions and improved convenience for the recording, archiving, processing, analysis and reconstruction of images. Topography, online measurement, physiology and digital high-speed recording modules have been added. Several features have been improved, including a wavelet algorithm that calculates the extended depth of focus. The software also can compensate automatically for the lateral displacement of Z-stack images taken with stereomicroscopes. An “inside 4D” mode allows the simultaneous display of transparency and surface render methods.