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Immersion Oil

Photonics Spectra
Dec 2009
Cargille-Sacher LaboratoriesRequest Info
Cargille Laboratories designed its immersion oil type 37DF to provide optical values at 37 °C for a sharp image. Immersion oil standardized for room temperature at 23 °C may cause a decrease in resolution when used at 37 °C because critical optical values of the oil change with temperature. The 37DF, as does the DF at 23 °C, offers very low background fluorescence, making it suitable for fluorescence microscopy applications. The oil is available in 1-, 4- and 16-oz bottles.


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The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
immersion oil
An oil required by oil-immersion objectives that is applied between the exterior of the objective lens and a cover glass or appropriate specimen.
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