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3-D Microscopy

BioPhotonics
Jan 2010
Andor Technology Ltd., Corporate HeadquartersRequest Info
 
A stochastic optical reconstruction microscopy (STORM) approach uses an iXonEM+ electron-multiplying CCD camera from Andor Technology and an inverted microscope from Olympus to resolve cellular structures with nanometer precision. Having a lateral (XY) resolution of 20 to 30 nm and axial (Z) resolution of 50 to 60 nm, along with a camera sensitive enough to detect single molecules, the STORM technology helps researchers see the 3-D relationship between mitochondria and microtubules. The ultrasensitive high-speed camera uses sequential imaging of single photoactivatable fluorophore molecules as they toggle between bright and dark states. Offering short exposure times, the microscopy system is suitable for studying cellular molecular processes.


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GLOSSARY
camera
A light-tight box that receives light from an object or scene and focuses it to form an image on a light-sensitive material or a detector. The camera generally contains a lens of variable aperture and a shutter of variable speed to precisely control the exposure. In an electronic imaging system, the camera does not use chemical means to store the image, but takes advantage of the sensitivity of various detectors to different bands of the electromagnetic spectrum. These sensors are transducers...
exposure time
The length of time during which the receiver is irradiated.
inverted microscope
A microscope designed so that the specimen is located face down above the objective.
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