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Immersion Oil

BioPhotonics
Jan 2010
Cargille-Sacher LaboratoriesRequest Info
 
Cargille Laboratories has designed type 37DF immersion oil to provide the optical values required for a sharp image at 37 °C. Immersion oil standardized for room temperature at 23 °C may offer decreased resolution when used at 37 °C because critical optical values change with temperature. The 37DF, as does the DF at 23 °C, offers very low background fluorescence, making it suitable for fluorescence microscopy applications. The oil is available in 1-, 4- and 16-oz bottles.


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GLOSSARY
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
immersion oil
An oil required by oil-immersion objectives that is applied between the exterior of the objective lens and a cover glass or appropriate specimen.
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