Scientific Volume Imaging BV has released version 3.5 of its Huygens 3-D deconvolution, visualization and analysis software. This version includes the newly developed object stabilizer tool that can measure and correct for cell motion in time series, or movements in Z-series that are caused by shaking or thermal drift. Stabilization methods based on cross correlation, multi-object tracking or center of mass alignment can be selected and involve X-Y-Z translation and axial rotation. New features include improved spherical aberration correction by taking into account the coverslip position and imaging direction, and an HDF5 file format reader and writer to store more microscopic parameters and data in a fully open, compact and easy to access format. The graphical interface has been modernized, scheduling of high-throughput deconvolution tasks is improved, and settings in visualization and analysis tools can be saved in templates.