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  • UV Laser

May 2010
Coherent Inc.Request Info
The Genesis CX355-250 laser from Coherent Inc. delivers true-CW, ultraviolet, solid-state performance, producing more than 250 mW of TEM00 output. It uses proprietary optically pumped semiconductor laser technology, providing a combination of <0.5% noise and high output power, which enables OEM instruments to achieve good signal-to-noise characteristics. The output beam has an M2 value of <1.2 to enable optimum collimation and/or refocusing to a diffraction-limited spot. Applications include flow cytometry of live cells, such as egg and fertilized-egg sorting, and high-throughput cytometers incorporating two parallel laser interaction cells. It also is suitable for other fluorescence-based applications such as confocal microscopy, where its short wavelength and low M2 beam provide optimum excitation of Hoechst and Indo dyes, and for 3-D prototyping applications, i.e., stereolithography.


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1. A bundle of light rays that may be parallel, converging or diverging. 2. A concentrated, unidirectional stream of particles. 3. A concentrated, unidirectional flow of electromagnetic waves.
1. The process of aligning the optical axes of optical systems to the reference mechanical axes or surfaces of an instrument. 2. The adjustment of two or more optical axes with respect to each other. 3. The process by which a divergent beam of radiation or particles is converted into a parallel beam.
flow cytometry
A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. Particles may be stained with fluorescent dye and the fluorescence detected via laser illumination.
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
A method of creating real three-dimensional models by using lasers driven by CAD software. In contrast to the normal practice of removing material, this process polymerizes a liquid to quickly produce shapes that are untouched by human hands or cutting tools. Also known as three-dimensional imaging and three-dimensional modeling.  
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