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Live-Cell Fluorescence Imaging

BioPhotonics
May 2010
Olympus Europa SE & Co. KG, Medical Systems & Micro-Imaging Solutions GroupRequest Info
 
Olympus Europa Holding GmbH has introduced its cell^FRAP system for fluorescence recovery after photo-bleaching (FRAP), fluorescence loss in photobleaching, pattern bleaching to simulate fluorescent speckle microscopy, photoactivation and photoconversion experiments. It performs millisecond real-time photocontrol and photomanipulation with hardware-based device synchronization. Intelligent scanning algorithms that target only the region of interest (ROI) remove the need for line scanning between ROIs and increase scan speed. Simultaneous photobleaching and image acquisition of multiple ROIs are possible. The system offers predefined FRAP experiments for investigation of cellular dynamics, and “fire on click” experiments enable online triggering of single- and multipoint bleaching. It uses a real-time controller and camera-based imaging.


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GLOSSARY
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
photobleaching
A process that helps optical fibers recover from damage induced by radiation. When silica is irradiated, bonds break and attenuation increases. Light in the fiber assists in recombining the species released by the broken bonds, decreasing attenuation.
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