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  • Scientific CMOS Camera

May 2010
Hamamatsu CorporationRequest Info
Hamamatsu Corp.’s ORCA-Flash2.8, a high-sensitivity digital camera based on the FL-280 scientific CMOS image sensor, is designed for low-light imaging at high frame rates. The FL-280 features 2.8 megapixels and a pixel size of 3.63 x 3.63 μm. Its wavelength sensitivity ranges from the UV to the visible, with peak sensitivity at ~450 to 500 nm and with >60% quantum efficiency. Typical readout noise is 3 e rms, even at fast readout speeds. Producing low dark current, it requires cooling to only 5 °C. The camera’s readout speed ranges from 45 fps at full resolution to 1273 fps with subarray readout. Applications include ratio imaging; Förster resonance energy transfer, fluorescence in situ hybridization and total internal reflection fluorescence microscopy; real-time confocal microscopy; semiconductor inspection; and industrial imaging.


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digital camera
A camera that converts a collected image into pixels that are black or white digital or shades of gray. The digital data may then be manipulated to enhance or otherwise modify the resulting viewed image.
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
Smallest amount into which the energy of a wave can be divided. The quantum is proportional to the frequency of the wave. See photon.
1. In optics, the ability of a lens system to reproduce the points, lines and surfaces in an object as separate entities in the image. 2. The minimum adjustment increment effectively achievable by a positioning mechanism. 3. In image processing, the accuracy with which brightness, spatial parameters and frame rate are divided into discrete levels.
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