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Structured Illumination

Photonics.com
Jul 2010
Leica Microsystems GmbHRequest Info
 
WETZLAR, Germany, July 26, 2010 — Resolution and contrast are important for fluorescence applications in wide-field microscopy. Undesired haze effects that reduce contrast can be minimized by using the smart principle of structured illumination. The result is fluorescence images with good contrast, high axial resolution and ultrasharp 2- and 3-D sections of the specimen.

Intelligent Structured Illumination Microscopy from Leica Microsystems GmbH uses the proven OptiGrid technique. Working in harmony with the company’s high-quality optics, the aperture diaphragm integrated with the fluorescence light path also enhances contrast. The automatic focus function of the OptiGrid keeps the grid structure in focus, from ultraviolet to infrared light, and produces high-resolution images at the press of a button.

The same OptiGrid module can be used for the company’s upright and inverted research microscopes, and there is no need to change the grid because one optimized grid covers all magnifications from 10× to 100×, which offers convenience, avoids errors and saves valuable time.

Intelligent Structured Illumination Microscopy provides the user with a complete system from a single source. The OptiGrid module integrated with the research microscopes is controlled by intuitive Leica MM AF software and complemented by a wide selection of fluorescence cameras.


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GLOSSARY
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
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