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pco.edge Scientific CMOS Camera

Photonics.com
Aug 2010
PCO-TECH Inc.Request Info
 
ROMULUS, Mich., Aug. 3, 2010 — The Cooke Corp. has introduced the pco.edge camera system that offers complete high-fidelity scientific imaging. Based on a scientific CMOS image sensor, it delivers low noise, fast frame rates, a wide dynamic range, high quantum efficiency (QE), high resolution and a large field of view, simultaneously and all in one image.

The camera features low readout noise of 1.4 e¯ rms. Even at the maximum speed of 100 fps at full resolution of 2560 × 2160 pixels (5.5-megapixel, 21.8-mm diagonal, 6.5-µm pixels) the readout noise is <2 e¯. Increased frame rates with smaller regions of interest (for example, 900 fps at 320 × 240 pixels) are user-selectable. In comparison to a 1.3-megapixel scientific CCD camera featuring 6 e¯ readout noise at 10 fps, the pco.edge camera at 1.3-megapixel resolution features <2 e¯ readout noise at a frame rate of 210 fps.

The low readout noise combined with the high full-well capacity of 30,000 e¯ achieves an intrascene dynamic range of better than 22,000:1, providing high dynamic 16-bit images.

Unlike electron-multiplying CCD image sensors that feature on-chip amplification to detect the lowest light levels at the expense of reduced effective QE, the camera achieves a peak QE of >55% without compromising spatial and temporal resolution, making it suitable for use in low-light conditions above two photons (zero dark current conditions).

User-selectable choice of rolling or global shutter mode for exposure provides flexibility for a variety of applications. The camera features 5 °C stabilized Peltier cooling, allowing for continuous operation free of any drift phenomena in image sequences.

Applications include live-cell microscopy, particle imaging velocimetry, single-molecule detection, super-resolution microscopy, total internal reflection fluorescence microscopy/waveguides, spinning disk confocal microscopy, genome sequencing (second- and third-generation), Förster resonance energy transfer, fluorescence recovery after photobleaching, lucky astronomy/imaging, adaptive optics, solar astronomy, fluorescence spectroscopy, bio- and chemiluminescence, high-content screening, photovoltaic inspection, x-ray tomography, ophthalmology, flow cytometry, biochip reading, machine vision, TV/broadcasting, spectral and hyperspectral imaging, and laser-induced breakdown spectroscopy.


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GLOSSARY
adaptive optics
Optical components or assemblies whose performance is monitored and controlled so as to compensate for aberrations, static or dynamic perturbations such as thermal, mechanical and acoustical disturbances, or to adapt to changing conditions, needs or missions. The most familiar example is the "rubber mirror,'' whose surface shape, and thus reflective qualities, can be controlled by electromechanical means. See also active optics; phase conjugation.
bioluminescence
Heatless light emissions from living organisms caused by the combination of oxygen and pigments such as luciferin.
camera
A light-tight box that receives light from an object or scene and focuses it to form an image on a light-sensitive material or a detector. The camera generally contains a lens of variable aperture and a shutter of variable speed to precisely control the exposure. In an electronic imaging system, the camera does not use chemical means to store the image, but takes advantage of the sensitivity of various detectors to different bands of the electromagnetic spectrum. These sensors are transducers...
chemiluminescence
A chemical reaction involving the production of light. The reaction of ethylene with ozone is chemiluminescent.
flow cytometry
A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. Particles may be stained with fluorescent dye and the fluorescence detected via laser illumination.
fluorescence spectroscopy
The spectroscopic study of radiation emitted by the process of fluorescence.
high-content screening
Also known as HCS, an analytical method designed to collect statistically relevant amounts of quantitative data on many parallel cell populations or processes within cells through the combination of microtiterplates, high-resolution imaging, automated microscopy, fluorescent or bright-field sensors and image analysis software.  
hyperspectral imaging
Methods for identifying and mapping materials through spectroscopic remote sensing. Also called imaging spectroscopy; ultraspectral imaging.
ophthalmology
The branch of medicine involved in the study of the anatomy, functions, diseases and treatments of the eye.
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