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LED Light Source

EuroPhotonics
Aug 2010
Carl Zeiss Microscopy LLCRequest Info
 
New LED technology has been incorporated into Carl Zeiss MicroImaging Inc.’s Colibri illumination system, a light source for wide-field fluorescence microscopy that uses wavelength windows with less need to suppress unwanted peripheral wavelengths from a white-light arc lamp. The system uses up to four LEDs, each individually and instantly controlled by electrical current without mechanical switching devices such as the filter wheels or shutters required by traditional illumination systems. Various colored LEDs can be used in combination, enabling users to see multiple fluorochromes simultaneously and to rapidly capture sequential images of each one.


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GLOSSARY
filter wheel
A device that holds a number of filters and allows the filter with the desired characteristics to be rotated into an optical aperture.
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
shutter
A mechanical or electronic device used to control the amount of time that a light-sensitive material is exposed to radiation.
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