BioTek Instruments Inc. has released the Synergy H1 hybrid multimode microplate reader, a monochromator-based instrument for ultraviolet/visible absorbance, top and bottom fluorescence, and luminescence assays. It can be easily upgraded to a hybrid reader with the addition of a filter-based optical module that includes its own light source and a dichroic-based wavelength selection system. This independent filter module with dichroic mirrors supports fluorescence polarization, time-resolved fluorescence, time-resolved Förster resonance energy transfer and bioluminescence resonance energy transfer assays with ultrahigh sensitivity. A dual reagent injection system is available to automate inject/read assays such as ion channels or flash luminescence. The monochromator optics use a third-generation quadruple grating design that enables luminescence detection and working at any fluorescence intensity.