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Epifluorescence Optics Modules

Photonics.com
Nov 2010
LabSmith Inc.Request Info
 
LIVERMORE, Calif., Nov. 5, 2010 — LabSmith Inc. has released the EPI epifluorescence optics modules for its SVM340 synchronous video microscope. They are designed for applications that require higher wavelength discrimination and/or improved signal-to-noise ratio, including microfluidics and biological applications such as cell imaging and microparticle image velocimetry.

The interchangeable modules extend the applications for the SVM340. Adding epifluorescence capabilities to the microscope lets researchers visualize and record flows using greater selectivity, limits of detection and resolution.

The microscope combines synchronous pulsed fluorescence illumination, sensitive video output, staging that will not perturb microsystems, and sequencing software for capturing events in microfluidics systems. Interchangeable optics modules and image capture and processing software shorten the learning curve for obtaining publication-quality images.

The company offers EPI-blue and EPI-green modules, suitable for imaging FITC and CY-3 conjugates, respectively. Each sealed EPI module is self-contained and includes a high-sensitivity camera, excitation and emission filters, a dichroic mirror, an LED illuminator and an objective mount. The microscope also features a four-channel external LED ring illuminator for increased intensity.


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GLOSSARY
camera
A light-tight box that receives light from an object or scene and focuses it to form an image on a light-sensitive material or a detector. The camera generally contains a lens of variable aperture and a shutter of variable speed to precisely control the exposure. In an electronic imaging system, the camera does not use chemical means to store the image, but takes advantage of the sensitivity of various detectors to different bands of the electromagnetic spectrum. These sensors are transducers...
dichroic mirror
A mirror used to selectively reflect light according to its wavelength and not its plane of vibration.
microscope
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
video
Referring to the bandwidth and spectrum location of the signal produced by television or radar scanning.
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