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Superresolution Microscope

Photonics Spectra
Jan 2011
Nikon Instruments Inc.Request Info
 
Nikon Instruments Inc. has released the N-SIM, a superresolution microscope that operates at speeds enabling the study of microstructures and nanostructures of fixed and living cells with molecular-scale resolution. Based on the Eclipse Ti inverted microscope, the instrument produces twice the resolution of conventional optical microscopes, combining structured illumination microscopy (SIM) technology – licensed to Nikon by the University of California, San Francisco – with the company’s CFI Apo total internal reflection fluorescence 100x oil objective lens that has a numerical aperture of 1.49. N-SIM achieves a time resolution of 0.6 s per frame and is effective for live-cell imaging.


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GLOSSARY
frame
1. To center an image or place it in any part of the television screen desired. Also applies to stills. 2. A single image of the connected multiple images on motion-picture film. 3. The size of the copy produced by a facsimile system. 4. In raster-scanned television, the combination of two interlaced field scans, making a single frame.
inverted microscope
A microscope designed so that the specimen is located face down above the objective.
lens
A transparent optical component consisting of one or more pieces of optical glass with surfaces so curved (usually spherical) that they serve to converge or diverge the transmitted rays from an object, thus forming a real or virtual image of that object.
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