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Flow Cytometry

BioPhotonics
Jan 2011
Beckman Coulter Inc., Diagnostics Div.Request Info
 
Beckman Coulter Inc. has introduced Krome Orange, a violet-excited organic dye that expands the range of choices and dye sensitivity limits for violet lasers. Common gating markers can be transferred to this fluorochrome, freeing other channels for use with more esoteric markers and enabling more versatile 10-color flow cytometry applications. It has excitation and emission maxima of 398 and 528 nm, respectively, and provides a brighter signal than Pacific Orange conjugates with equivalent spectral overlaps into adjacent channels. The dye enables scientists to increase sensitivity for low fluorescence signals and to expand their flexibility for multicolor flow cytometry experiments.


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GLOSSARY
excitation
1. The process by which an atom acquires energy sufficient to raise it to a quantum state higher than its ground state. 2. More specifically with respect to lasers, the process by which the material in the laser cavity is stimulated by light or other means, so that atoms are converted to a semistable state, initiating the lasing process.
flow cytometry
A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. Particles may be stained with fluorescent dye and the fluorescence detected via laser illumination.
organic dye
Any organic substance, that when dissolved in appropriate liquid based solvents will absorb and emit electromagnetic radiation in the near ultraviolet, visible and near infrared spectral regions. These dyes are suitable gain mediums for the dye laser. See organic dye laser.
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