The ProScan III scanning stages from Prior Scientific Inc. now are compatible with Carl Zeiss MicroImaging GmbH’s AxioVision microscope software. Motorized scanning stages occupy a central position in microscope automation. Besides high repeatability and precision, smoothness of operation and long-term stability are important requirements for X-Y stages and their control electronics. The ProScan III scanning stages are used in high-end microscopy applications. With the release of the Zeiss AxioVision 4.8.2 and MicroToolBox version 1.8.1, AxioVision users can select Prior’s scanning stages in combination with the ProScan III stepper motor controller. In collaboration with Zeiss, Prior developed controller firmware to enable direct stage programming through AxioVision.