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  • Chameleon Vision-S Laser
Feb 2011
Coherent Inc.Request Info
SANTA CLARA, Calif., Feb. 28, 2011 — The Chameleon Vision-S ultrafast laser from Coherent Inc. is used for multiphoton imaging and combines 80-fs pulse widths with high average power and a long wavelength tuning range.
It delivers more than 410 kW of usable peak power, after group velocity dispersion precompensation (GVD), and hands-free tuning from 690 to 1050 nm. The blend of short pulse widths and high average power translates into high precompensated peak power from a one-box laser.

The laser is suitable for multiphoton excitation imaging for several reasons. Its high peak power delivers maximum imaging brightness in deeper tissues and, because it is a true one-box laser with the laser oscillator and GVD precompensator integrated on a single baseplate, it also provides good beam pointing stability of 80 µrad per 100 nm of tuning, which is another prerequisite for deep tissue imaging.

The GVD precompensator provides seamless, hands-free adjustment of negative dispersion from zero up to a maximum of 43,000 fs2 to enable good pulse duration flexibility at the sample, even in next-generation microscopes with multiple acousto-optic modulators. These next-generation microscopes provide fast scanning of selected image sectors in applications, such as neuroscience, that require high frame rates to follow dynamic events in real time. The circular TEM00 Gaussian output beam with M2 <1.1 maximizes image resolution at any tissue depth.


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