The SymPhoTime version 5.2.4 fluorescence lifetime imaging and correlation software from PicoQuant GmbH supports the eight-channel configuration of the company’s HydraHarp 400 time-correlated single-photon-counting system and, therefore, data acquisition and analysis for up to eight detection channels. Another enhancement is the extension of the measurement preview functions. It is now possible to display time-correlated single-photon-counting histograms as well as intensity time traces for each channel separately during measurement. This is advantageous for Förster resonance energy transfer measurements because it enables direct monitoring of changes in the fluorescence intensity of the donor and acceptor during data acquisition. The online fluorescence lifetime imaging microscopy features of the software also have been improved by such additions as scalable intensity and a lifetime color code.