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BZ-9000 Fluorescence Microscope

Photonics.com
Jun 2011
KEYENCE Corp. of AmericaRequest Info
 
WOODCLIFF LAKE, N.J., June 14, 2011 — The BZ-9000 BioRevo from Keyence Corp. of America offers a new approach to conventional fluorescence microscopy.

The fully integrated system performs fluorescence, bright-field and phase-contrast imaging on a variety of specimen holders, including slides, dishes and well plates, without the need for a darkroom. Integrating a motorized, six-objective lens revolver, a motorized stage, an electronically controlled filter turret and a built-in sample enclosure reduces the amount of tabletop space required for a conventional fluorescence microscope by more than 40%, according to the company.

The microscope switches between up to four fluorescent channels with a click of the mouse, automatically adjusting the filter and exposure time and reducing the amount of time needed to perform research and analysis, while preserving the longevity of the specimen.

Although conventional systems require a user to manually switch between monochrome and color imaging modes, usually with two cameras, the BZ-9000 incorporates a single high-resolution, high-sensitivity 12-bit monochrome camera that can easily switch to color imaging with the click of a mouse. The navigation system allows users to easily locate areas of interest on a specimen when at high magnification by using a low-magnification image for reference.

The instrument overcomes limitations associated with traditional fluorescence microscopes by incorporating a Z-stack and image stitching function. Users can stitch up to 1200 images in X-Y directions to capture a large field of view, even when at high magnification. A quick full focus and Z-stack function captures fully focused images in a matter of seconds.

The BioRevo provides options for quantitative analysis, including standard 2-D measurements, cell counting and brightness analysis. A real-time 3-D module generates a 3-D image of a fluorescent specimen, eliminating some of the reliance on laser confocal systems.

Because the unit has a built-in sample enclosure, an option also is available for conducting live-cell and time-lapse imaging with the use of an incubation chamber.


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GLOSSARY
color
The attribute of visual experience that can be described as having quantitatively specifiable dimensions of hue, saturation, and brightness or lightness. The visual experience, not including aspects of extent (e.g., size, shape, texture, etc.) and duration (e.g., movement, flicker, etc.).
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
microscope
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
monochrome
Made up of a single color.
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