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Visi-Blue Technology

Photonics.com
Jul 2011
UVP LLCRequest Info
 
UPLAND, Calif., July 29, 2011 — UVP LLC has announced enhanced visualization and imaging capabilities with new Visi-Blue technology. Visi-Blue transillumination is recommended for viewing a wide range of fluorescent stained gels that emit light between 460 and 470 nm, including GelGreen, SYBR Green, SYBR Gold, SYBR Safe, GelStar, SYPRO Ruby and SYPRO Orange. The technology, including transilluminators and converter plates, does not produce UV light and therefore eliminates UV exposure and minimizes damage to samples. It allows researchers to perform experiments without concern about harmful UV effects.

The combination of Visi-Blue instruments’ lighting and specially designed filter glass enables bright gel illumination and excitation. The light reduces background noise and increases the image signal, creating higher contrast for enhanced visualization and analysis. An amber cover, included with the transilluminators and optional on the converter plates, absorbs the blue excitation and provides a clear view of the stained gel’s brilliant fluorescence.

When viewing the fluorescent gel through the amber cover, researchers can easily see the critical aspects of the sample, allowing for optimal band cutting. For those who currently use a UV transilluminator, the Visi-Blue converter plate converts UV to a safe 460- to 470-nm excitation wavelength. The instruments are available in a variety of filter sizes to accommodate gels ranging from 21 × 26 to 20 × 40 cm.

For researchers photographing gels using Visi-Blue instruments with a proprietary gel imaging system, the company supplies its own camera filter with all equipment. Captured gels clearly display high sensitivity.

The technology allows laboratory researchers to use a variety of newer and safer stains for fluorescence imaging. The stains are safer than ethidium bromide, a known carcinogen and mutagen. Eliminating it from the lab increases user safety, decreases hazardous material control costs, and virtually eliminates mutagenic and UV damage to samples.


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