PicoQuant GmbH has unveiled 500- and 510-nm picosecond pulsed diode laser heads. Green laser emission is not generated by wavelength conversion techniques but directly by a semiconductor laser diode. The new wavelengths open the door for applications in bioanalytics, biochemistry, genetics, semiconductor characterization and quality control. The laser heads are suitable for exciting fluorescent proteins such as EYFP, mCitrine and Venus, and for widely used fluorescent dyes such as Alexa 514, Atto 520 and Oregon Green. They also fill a demand for alternatives to frequency-doubled solid-state lasers and argon-ion types in applications such as fluorescence microscopy. With pulse durations as short as 130 ps, the laser heads match the time resolution of mainstream detectors at a price lower than that of mode-locked lasers.