Princeton Instruments’ controllerless, cryogenically cooled CCD cameras are designed for quantitative spectroscopy applications that demand high sensitivity. The first commercially available models are the PyLoN:100 and PyLoN:400 dual-amplifier cameras, which use 1340 x 100 and 1340 x 400 sensors, respectively. In creating the new platform, the company redesigned its Spec-10 family of cameras to remove the external controller, thus increasing experimental flexibility and improving the ultralow-noise electronics. Liquid nitrogen cooling virtually eliminates dark current, and indium metal seals enhance vacuum longevity. Binning noise has been reduced. The amplifiers and software-selectable gain permit operation in high-sensitivity mode for Raman or single-molecule spectroscopy, and in high-capacity mode for fluorescence spectroscopy. The PyLoN:100 is suitable for rapid spectral acquisition and the PyLoN:400, for multiplexed spectroscopy.