For live-cell imaging applications, Leica Microsystems has unveiled the DFC365 FX digital microscope camera. To document live cells and rapidly fading fluorescence specimens, it combines high-quality imaging with high temporal resolution for rapid time-lapse recordings. It enables researchers to work efficiently, even with weakly fluorescing specimens. Equipped with a sensitive CCD sensor with 6.45-µm pixels and active Peltier cooling, it is suitable for applications ranging from basic fluorescence documentation to total internal reflection fluorescence, Förster resonance energy transfer and structured illumination. It operates at acquisition rates of 21 fps at full resolution. In “overlapping mode,” an image can be captured while the previous image is still being read out. Data is transferred to the PC via a FireWire-B interface.