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25x Objective Lens

Photonics.com
Dec 2011
Olympus Europa SE & Co. KG, Medical Systems & Micro-Imaging Solutions GroupRequest Info
 
HAMBURG, Germany, Dec. 22, 2011 — Olympus Europa Holding GmbH has released the XLPLN25XSVMP ScaleView 25× objective lens with a numerical aperture of 1.0 for deep imaging of thick biological samples.

The ScaleView approach allows researchers to create accurate 3-D structural representations of brain tissue. Until now, the use of such samples has been limited by the effects of tissue opacity and light scattering. To counter this, the new objective has a long working distance of 4 mm and works alongside Olympus’ ScaleView-A2 clearing agent, which renders samples nearly translucent while preserving fluorescent signals.

Image quality, sharpness and brightness are then maximized as the objective is optimized to match the refractive index properties of the clearing agent. When used as part of a complete Olympus FluoView FV1000MPE multiphoton system, the new objective makes it possible to peer deeper into samples, allowing the user to generate insightful results from intact specimens.

The lens and clearing agent are designed to boost the capabilities of multiphoton microscopy, allowing accurate reconstructions up to a depth of 4 mm to be generated using fluorescent markers. By clearing formalin-fixed tissues and allowing light to pass through the sample, the ScaleView-A2 reagent minimizes the need for sectioning, allowing the user to generate data that more accurately reflects the true internal structure of a complex specimen. Using the technique, true 3-D representations can be created, without the need for complex interpolation, predictive algorithms or guesswork.

The objective is equipped with a correction collar and is optimized to work best with the A2 reagent (refractive index 1.38), facilitating production of detailed, crisp images. As part of a complete system, the ScaleView approach integrates seamlessly with Olympus’ FluoView FV1000MPE multiphoton microscopes and the FV10-ASW software v3.1, providing the power to visualize 3-D structures in morphologically intact tissue.


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