BOULDER, Colo., May 22, 2012 — REO Inc. has released a 594-nm (yellow) HeNe laser suitable for bioinstrumentation and fluorescence excitation applications. It features a circular beam profile and beam quality with M2 <1.05, resulting in minimized loss in single-mode fiber coupling. It produces polarization purity of >500:1, with a guaranteed minimum output power of 2 mW. The yellow lasers provide noise output of <1% from 30 Hz to 10 MHz and good beam stability. Long-term beam drift is <0.05 μrad. They are packaged in an industry-standard cylindrical head format measuring 44.5 mm in diameter, and the front plate supports a variety of fiber coupling options. The company offers a choice of OEM or Center for Devices and Radiological Health-compliant power supplies, including time delay, key switch and interlock. The gain in HeNe is weak at 594 nm, so achieving stable laser output at this wavelength requires high-quality cavity mirrors with low scatter and precision coatings that reject nearby laser lines. These must then be assembled onto laser tubes in a low-contamination environment. REO produces its own laser tubes, mirror substrates and coatings in-house, using techniques such as superpolishing and ion-beam-sputtering deposition to produce mirrors with high reflectance, low scatter, environmental stability and mechanical durability. The lasers meet a growing demand for yellow wavelength excitation in fluorescence-based bioinstrumentation applications such as confocal microscopy, flow cytometry and drug discovery, as well as emerging applications in proteomics and genomics. The yellow output is suitable for use with fluorophores such as Texas Red and Alexa Fluor 594, and with APC (allophycocyanin) and its tandems. These applications benefit from the yellow HeNe’s round beam profile and stable output beam, eliminating the spatial filtering and collimation optics often required with other laser types.